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However, the short scale (minute to minute) time course of ethanol induced changes of zebrafish behaviour has not been analyzed.
Δhly (LLO-minus) L. monocytogenes and heat-killed bacteria stimulated almost no LC3-I to LC3-II conversion, as compared to wild-type L. monocytogenes over a 60 minute time course of infection (Fig. 1B and data not shown).
As depicted in Figure 4A (inset), a drop of Tris buffered solution dispensed onto a hydrophobic surface exhibited very little surface spreading over a 45 minute time course, with some loss of volume due to evaporation.
Western blots from control cells which received fresh media only (no PE) during the 60 minute time course revealed that media alone did not induce significant activation of the growth pathways examined (Fig. 2A D).
Accumulated dye in the region of the sentinel lymph nodes was imaged to a depth of 10-12 mm over a 10 minute time course.
The lack of effect of A-803467 on spontaneous activity was consistent across the 15 minute time course perineurium (P = 0.46 two-way ANOVA; n = 4/group; Figure 3).
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Therefore, we assayed activation of the mutant protein as compared to the wild type protein over a 45-minute time course.
The PVT was used to assess mean reaction time over a 5-minute time course.
The responses were measured at different concentrations over a 3- to 30-minute time course.
LPCAT assays were conducted at pH 7.4, with shaking at 100 rev/min in a water bath at 30°C in a 20-minute time course.
The incorporation of FM1-43 dye into MKs during a 20-minute time course indicates that vesicular endocytosis takes place within MKs.
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