Upon completion of the 10 minute testing period, the location of the adult was scored by a blind observer as either the EA arm, ambient air arm, or no choice.
Upon completion of the 10 minute testing period, larval location was scored by a blind observer as either the EA arm, ambient air arm, or no choice (defined as the loading arm or any portion of the central chamber); larvae were then placed in separate rearing boxes based upon their choice of arm, and remained there until pupation.
Our stressors yielded inconsistent responses, but the average values over the 30 minute testing period were useful for assessing treatment effect.
We then measured the length of time that fasted male mice spent investigating a dried vanilla spot painted on the inside of the cage, during a five minute test period.
This difference between strains was consistent over the 5 minute test period.
The total number of entries into the inner zone of the open field over the 5 minute test period was significantly greater for SHR compared to WKY rats (Kruskal-Wallis H (3, N = 92) = 64.5, p < 0.0001, Mann-Whitney U test, p < 0.0001, Figure 2b).
Noradrenaline, adrenaline, dopamine, plasma-free serotonin, platelet serotonin, glucose, glucagon, and insulin were measured throughout the 180-minute testing period.
10, 11 Noradrenaline, adrenaline, dopamine, plasma free serotonin (f5-HT), platelet serotonin (p5-HT), glucose, glucagon, and insulin were measured throughout the 180-minute testing period.
The test was run and videotaped for the last 5 minutes of the 6 minutes testing period.
For example, meditation, even in the novice group, appeared to help maintain PVT performance across the entire 10 minute test periods (in the first post-test but not the one in the second hour), whereas performance declined following nap or control periods within these single 10 minute tests.
The temperature change and evaporation rate were recorded throughout a 90-minute test period.
