The method presented here identifies peak events occurring at or above the 99th percentile of by-minute suspended particulate data.
It all comes down to which minute particles, or particulate matter (PM), in the air are being measured.
The survey instruments produced minute readings of particulate matter less than 10 microns, 2.5 microns and 1 micron in diameters (PM10, PM2.5, and PM1 respectively) and computed readings of total suspended particulates (TSP), as well as humidity, barometric pressure, wind speed and wind direction.
Then, coconut water (TCW) was extracted and centrifuged at 4000 rpm for 20 minutes to separate particulate matter, followed by filter sterilization with 0.22 μm filter (Millex® filter units – Millipore).
Some, such as colonial corals with minute polyps, feed on particulate material gathered in mucus impelled to the mouth by cilia (microscopic hairlike projections of cells capable of beating or waving).
Taking into account chamber TSP levels, exposure duration, mouse BW, minute ventilation, and total particulate matter released from the burning of a single cigarette, this exposure level was roughly equivalent to smoking about one pack of cigarettes per day.
After various treatments, serum-free conditioned media samples were collected and centrifuged at 10,000 × g for 5 minutes to remove particulates.
The powdered extract was suspended in sterile water to a stock concentration of 25 mg/mL (w/v), centrifuged at 10,000 × g for 20 minutes to remove particulates and then filtered through a 0.2 μm nylon filter.
One-minute average concentrations of ultrafine particulate matter (UFP), fine particulate matter [≤ 2.5 μm in aerodynamic diameter (PM2.5)], and particle-bound polycyclic aromatic hydrocarbons were measured using a mobile-monitoring protocol.
Luminescence indices were calculated by dividing the mean luminescence counts per minute (cpm) of four replicate particulate-treated wells by the mean cpm of four negative control wells at each time point.
