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The program used was as follows: 1 minute of initial denaturation at 94°C, followed by 30 cycles of denaturation (94°C for 30 seconds), annealing (56°C for 30 seconds) and extension (72°C for 1 minute) with a final extension for 7 minutes at 72°C.
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Thirty minutes of initial perfusion preceded baseline data acquisition.
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She should be throwing up into a bucket within 10 to 20 minutes of initial nudity.
After 30 minutes of initial fixation, a small incision was made on the cornea for better fixative penetration.
After two minutes of initial measurements, sodium benzoate was added to a final concentration, with no change in extracellular pH.
Adaptation and habituation patterns of human auditory evoked P1-N1-P2 responses halsoalso been examined using stimulus repetition paradigms and it is well documented that the N1 component shows rapid declines in amplitude, within minutes of initial stimulation [25], [40], [41].
In a porcine model of prolonged cardiac arrest, even four minutes of initial poor quality of CPR compromises the hemodynamics and survival outcome.
In this porcine model of prolonged cardiac arrest, even four minutes of initial poor quality of CPR compromises the survival outcome.
To minimise daily weight variations, participants are measured at a similar time of day (within 60 minutes of initial assessment) at all 3 assessment points.
Supporting this result, Jackson et al showed improvement in facial erythema within 30 minutes of initial daily application of brimonidine tartrate in Phase III clinical studies.
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