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They were instructed to limit movement during baseline and task measurements, and were verbally directed to close or open eyes for each minute of baseline recording.
Blood samples for hemoconcentration and hemostasis measures were collected during the last minute of baseline, math, and recovery.
One minute of baseline was collected at the end of the 15 minutes in the assigned condition, that is following 15 minutes of KMC or in incubator.
For each animal, the amplitude of normal baseline activity was determined as recurrent bursting of at least three bursts for any given minute of baseline.
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Following 30 minutes of baseline recordings, animals were injected i.p. with MK-801 or saline and recorded at 40 to 60 minutes post-injection corresponding to maximal drug response.
Each recording consisted of 5 minutes of baseline measurement, 5 minutes of occlusion measurement, and 5 minutes postocclusion measurement (hyperaemic period) as is showed in the Figure 1.
After loading the cells, two minutes of baseline fluorescence were was collected, and then cells were treated with EGTA (10 mM), ionomycin (10 µM), or 3O-C12 HSL.
Drugs were applied in the bath (except BAPTA, applied intracellularly), after recording 10 minutes of baseline and 10 minutes before cellular conditioning protocol, and were present continuously until the end of the recording.
After a few minutes of baseline recording, injections were performed into the vMPFC.
Following the collection of five minutes of baseline data, a blood pressure cuff on the upper arm was inflated to a suprasystolic level for five minutes.
After placing the optodes and TCD probes and after optimizing the optical and ultrasound signals, five minutes of baseline data were obtained.
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