The samples were rinsed by 3-one minute washes in cold Sorensen's buffer, followed by 15 minute post-fixation in cold 1% OsO4, and rinsed again.
Eggs - boil in a pan for several minutes - take in cold lunch bag, peel open and eat.
Tissue was permeabilized in 0.2% Triton in PBS for 10 minutes, followed by washing in cold PBS for 5 minutes.
Blanch collards in boiling water for 1 minute, then immediately place in cold water.
Soak the gelatine for five minutes in cold water.
Soak the chopped onion first for five minutes in cold water, then drain and rinse with cold water.
To get the most out of them, they need to be rehydrated: soak them for 10 minutes in cold water, drain, then sauté gently in oil and a splash or two of more water, until they begin to swell.
Small amounts of hair (up to one cm) have very little effect on the overall temperature of the skin after twelve minutes in cold temperatures, but once hair reaches a thickness of two cm, the head becomes significantly insulated and skin temperatures are much higher.
Minor treatments include "inhalation therapies," or people breathing into tubes, that once again summon my mother's credo, so I go for the one that seems most innocuous, a quarter-hour self-administered therapy in which I stand up to my knees for a minute in hot water, a half-minute in cold water, and switch back and forth.
Briefly, young adults were washed from growth medium in M9, rinsed and then fixed for 10 minutes in cold methanol (−20°C).
