Sentence examples for minute extension step from inspiring English sources

PCR consisted of 45 cycles of amplification (20 seconds at 95°C; 30 seconds at 58°C, 30 seconds at 72°C), followed by a 15 minute extension step at 72°C in an iCycler Thermal Cycler (Bio-Rad, Australia).

After reverse transcription of RNA, cDNA was subjected to PCR cycling conditions as follows: initial denaturation at 95°C for 2 minutes, 30 cycles of amplification consisting of a 15 s denaturation step at 95°C, a 30 s annealing step at 58°C, and a 1 minute extension step at 72°C.

A final 5-minute extension step was added after the last cycle.

A final 10-minute extension step was added after the last cycle to extend incomplete fragments.

After a heat-denaturation step, each of the 25 cycles of the subsequent PCR steps consisted of a 15-s denaturing step at 94°C, a 30-s annealing step at 59°C, and a 1.5-minute extension step at 72°C.

PCR amplification was performed using ABI thermocycler and the reactions were subjected to 35 PCR cycles of 94°C for 30 seconds, 60°C for 30 seconds, and 70°C for 30 seconds followed by 7 minutes' extension step at 72°C.

For zot gene, PCR amplification conditions described by Rivera et al. [ 24] were used and included an initial denaturation at 94°C for 2 minutes, denaturation at 94°C for 1 minute, annealing at 60°C for 1 minute, an extension step at 72°C for 1 minute at the end of 30 cycles and a final extension step at 72°C for 10 minutes.

For MATP and ASIP, programs consisted of an initial denaturation step at 95°C for 3 minutes, followed by 50 cycles of 20 seconds of denaturation at 95°C, 30 seconds of annealing at 55 57°C and 30 seconds of extension at 72°C, and completed by a 4 minute final extension step at 72°C.

Samples were heated to 95°C for 2 minutes before cycled according to table 1 at 95°C for 30 s, 60°C for 30 s and 72°C for 30 s before a 5 minute final extension step at 72°C on an MJ Research thermocycler.

The amplification profile was: activation of AmpliTaq Gold for 9 minutes at 95°C, denaturation for 1 minute at 95°C, annealing for 1 minute at 55°C and extension at 72°C for 1 minute, for a total of 40 cycles, followed by a 5-minute terminal extension step at 72°C.

The thermal profile was as follows: initial 5 minutes' denaturation step at 94°C for one cycle followed by repeating cycles of denaturation (30 seconds at 94°C), annealing (45 seconds at 55°C), and extension (40 seconds at 72°C) for 35 cycles, followed by a 5 minutes' final extension step at 72°C.