Between 30 and 120 minutes after sucrose addition, the expression level of suxA was 2 fold higher in the wild-type strain background than in the suxA deletion mutant background, although after 360 minutes, the expression levels were identical in both strains.
PP injection significantly reduced ARC GAD65 mRNA expression within 30 minutes compared to expression levels in saline-injected mice (Figure 4B+D, Table 2).
Second, their expression levels can be measured reliably in FFPE tissue samples, and only minute quantities of RNA are needed to establish their expression using reliable, quantitative PCR amplification strategies [ 33].
Although the expression patterns of these genes were equivalent in ΔVNG0258H and the parent for the first 40 minutes following H2O2 exposure, expression levels remained elevated compared to the parent level for the duration of the time course.
Furthermore, in normal B cells, all investigated phospho-proteins except p-S6, showed higher expression levels at 4 minutes compared to 45 minutes after BCR activation.
Down-regulation of the transcripts clustered in c0 started after the first minute displaying a sharp decrease in the expression levels after the first hour.
In detail for example, the elu dataset [ 15] involves 14 time points for a cell cycle, and the cdc15 dataset [ 15] involves 24 time points for three cell cycles (i.e., 8 time points per cell cycle); In the elu dataset, gene expression information was collected at every 30 minutes, while in the cdc15 dataset, gene expression levels were collected at every 10 minutes.
The Hirata data comprises scaled hes1 mRNA expression levels every 30 minutes over a 12 hour period; see the discrete points in Figure 1.
Further, the hcnABC genes did not exhibit a change in expression levels at 60 minutes, indicating resumption of aerobic metabolism.
Following 30 minutes of tensile strain β-catenin expression levels were comparable to those in the untreated cells (P = 0.196; Figure 1A)).
