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Arrays were washed for 1 minute each in 6× SSPE, 0.005% N-Lauroylsarcosine at room temperature and then in 0.06× SSPE, 0.005% N-Lauroylsarcosine at 37°C.
Slides then were dehydrated for 1 minute each in 70%, 85%, and 100% ethanol and air-dried.
Slides were dehydrated for 1 minute each in 70% EtOH, 85% EtOH, and 100% EtOH and then air dried.
Dehydration of slides was performed via an ethanol series: 1 minute each in 70% EtOH, 85% EtOH, and 100% EtOH, followed by air-drying.
Following hybridization, array slides were washed at room temperature for 1 minute each in 2X SSC/0.1% SDS, 1X SSC, 0.2X SSC, and 0.05X SSC and then dried by centrifugation.
The stained sections were dehydrated through increasing concentration (30%, 50 % and 70) of ethanol for 5 minutes each while for 1 minute each in 90% and 100% ethanof, clethanoln xylene, cover slipped with distrene plasticizer xylene (DPX) and air dried before observing under microscope for cytomorphometric analysis.
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