Sentence examples for minute cells were from inspiring English sources

After adding 400 ul of cold FBS for 1 minute, cells were washed 2× with 10 ml of PBS and resuspended at 1×106 cells/ml in RPMI-10.

An initial study on chimeric mice described that Minute cells were also eliminated from mouse embryonic tissue, but mechanistic insight was limited [ 15].

After centrifugation at 2000 round per minute, cells were lysed in RIPA buffer (Tris 1.5 M pH 8, NaCl 5 M, NP40 10%, DOC 10%, SDS 10%) with 0.5 mM DTT and 1/50 of complete protease inhibitor cocktail tablets (Roche, Neuilly sur Seine, France).

After 15 minutes, cells were washed in 1xPBS and imaged immediately.

After these 15 minutes, cells were washed with 1×PBS, fixed with 4% PFA, and processed for immunofluorescence.

After 15 minutes, cells were stimulated with 50 uM forskolin or DMSO alone for 30 minutes at room temperature and assayed for cAMP levels.

After cells were fixed for 20 minutes, cells were gently washed twice with PBS, and stained with 0.001% toluidine blue (Sigma-Aldrich Corp).

Following washing at 1600 rpm for 5 minutes, cells were poured into another tube through a 40 µm cell strainer, washed and used at 5×105/well.

After 30 minutes, cells were washed twice in HPMI containing 4% (w/v) fatty acid free bovine serum albumin to extract NBD lipids from the cell surface.

After staining for 30 minutes, cells were washed with PBS, and fluorescence intensity at 543 nm was measured on a COULTER EPICS XL Flow Cytometer (Beckman Coulter).

Following incubation with tetramers and mAbs for 20 minutes, cells were washed twice and analysed by flow cytometry using a FACSCanto in combination with FACS Diva software (BD).