Not 15 minutes before reading Merkin's essay, I came across an article about aging female gorillas at the Brookfield Zoo in Illinois.
It takes a few minutes before reading some geochemical parameters; this depends on the activation of external devices like electrovalves, pumps, etc.
The two RDT dipsticks were placed in the tubes and left for 10 to 15 minutes before reading.
The most commonly observed errors were a failure to wait 20 minutes before reading the test result, and adding an incorrect volume of buffer to the test cassette.
In order to avoid misreading by foam formation, milk samples were then slowly poured into a volumetric glass cylinder graduated to 5 ml and allowed to stand 5-10 minutes before reading.
The resulting mixture was gently agitated until it became a clear pink solution, after which we added the test strip to the solution and allowed it to stand for 25 minutes before reading the result.
200 μL Chromagen (ABTS) (Sigma-Aldrich Inc., USA) was then added to these wells and the mixture left to react at 25°C for 6 minutes before reading the absorbance at 750 nm using the Bio-Rad Benchmark Plus Microplate Reader.
Dual-Glo Luciferase reagent was then added into each well and incubated at room temperature for 10 minutes before reading the firefly luminescence generated using the GloMax Multi Detection System (Promega, USA).
2,2'-azino-di-3-ethylbenzthiazoline-6-sulphonate (KPL, Gaithersburg, Maryland, USA) was used as a chromogen and added to each well 15 minutes before reading optical density (OD) by the VMax microplate reader at 405 nm with a reference filter between 620 to 650 nm (Molecular Devices MDS Analytical Technologies, Sunnyvale, California, USA).
A 100 μL volume of 1 × Tris-EDTA was added to each well and then the plate was vortexed for 5 minutes before reading on the Luminex100 xMAP™ instrument (Luminex, Toronto, ON) following the manufacturer's instructions and as described in [ 59].
