Sentence examples for minute before mounting from inspiring English sources

It is advantageous to put one or two drops of cyanoacrylate glue on the cutting platform one minute before mounting the brain, to allow the glue to dry slightly.

In each of their last two games — victories against the Bills and the Redskins — the Jets sleepwalked for 55 minutes before mounting game-winning drives.

The slides were incubated with CAD Antibody Detection Kits Applied Spectral Imagingg, Migdal Ha'emek, Israel) at 37°C for 45 minutes and washed in 4× SST at 45°C for 5 minutes before mounting in Vectashield (Vector Labs, Burlingame, CA) with DAPI.

Cells were then washed with PBS, incubated with FITC-conjugated anti-rabbit secondary antibodies (DAKO) in fluorescence dilution buffer at 4°C for 1 hour and with Alexa Fluor 488 Phalloidin (Molecular Probe) at RT for 20 minutes before mounting.

Finally nuclear staining was performed with DAPI for three minutes, before mounting.

Cells were washed in buffer A and fixed in ice-cold 2% PFA for 15 minutes before mounting and imaging.

For Nile Red stains, larvae were dissected on a glass slide in 0.001% Nile Red (Sigma), 75% glycerol and incubated for 10 minutes before mounting.

Small skin pieces (2 × 2 cm) were incubated in PBS for 30 minutes before mounting the skin samples with the stratum corneum side uppermost.

After the staining step, the slides were dehydrated by successive incubations in 96% ethanol for 2 minutes, 100% ethanol for 2 minutes and xylene for 5 minutes, before mounting with Cytoseal 60 (Thermo Scientific).

Cells were allowed to migrate or invade for 24 hours and fixed in methanol for 20 minutes at -20°C, stained with haematoxylin, washed with dH2O and allowed to dry before mounting them in a microscopic slide with DPX (Sigma Aldrich).

For phalloidin staining, cells were incubated for 15 minutes in a 1 250 dilution (from 6.6 µM stock) of phalloidin-rhodamine (Life Technologies) just before mounting.