Sentence examples for minute before fixation from inspiring English sources

24 hours post-transfection cells were washed and permeabilised in the following ways: Selective permeabilisation of the plasma membrane was obtained by treating cells with 40 µM digitonin in KHM buffer for one minute before fixation using 4% formaldehyde in PBS.

For colcemide and taxol experiments, 1 3 h old embryos were treated with colcemide (5 mM) or taxol (25 µM) for 10 minutes before fixation as described previously [42].

After bath application of NMDA, culture medium was exchanged and neurons were incubated for additional 25 minutes before fixation.

Briefly, HeLa cells were incubated with ice cold media for 10 minutes before fixation and immunostaining for α-tubulin.

Human PBMC cultured on eight well glass microscope slides were air dried for at least 60 minutes before fixation in acetone for 15 minutes at room temperature.

In some cases, the cells were treated with actinomycin D (5 to 7.5 µg/ml) for 15 minutes before fixation or adhesion to slides.

For actin disruption experiments, transfected cells were treated with 10 µm latrunculin A (Sigma-Aldrich) in media for 10 minutes before fixation.

For lipid transport experiments, cells were labeled with 5 µM BODIPY TR Ceramide-BSA complex in DMEM (Molecular Probes, USA) for 40 minutes, then the extracellular fluorescent probe was eliminated by extensive washing with cold PBS, and finally, cells were incubated at 37°C for 30 minutes before fixation in 0,03% defatted BSA enriched cell culture medium.

For cold-stable microtubule assay, cells were placed on ice for 10 minutes before fixation.

Sorted cells were allowed to adhere to μ-Slide eight-well chamber poly-lysine-coated slides (Ibidi, Uddingston Glasgow, UK) for 15 minutes before fixation in 4% paraformaldehyde.

Incubation after copper addition continued for another 40 minutes before fixation in 4% paraformaldehyde for 1 hour at ambient temperature in the dark.