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The wet etching speed was about 40 Å per minute at room temperature.
One unit of POD activity was defined as the change in absorbance of 0.01 per minute at room temperature.
The cells were left for 7 minute at room temperature.
This was followed by an acetonitrile wash for 1 minute at room temperature and Stabilization and Drying Solution for 30 seconds at room temperature.
The membrane was neutralized in 0.5 M NaCl and 0.5 M Tris [pH 7.5] for 1 minute at room temperature, and then in 2X SSC.
Four microliter droplets of water, 100 µg lysozyme/ml water (Fisher), 100 µg native cSPLUNC1/ml water, or SDS (10%) (Fisher) were thereby incubated on the hydrophobic surface of Parafilm M for one minute at room temperature as reported [36].
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Let rest for 15 minutes at room temperature.
Leave to stand for 30‑60 minutes at room temperature.
Endogenous peroxidase activity was blocked using peroxidase 3% for 30 minutes at room temperature.
The complete polymerization of acrylamide/bisacrylamide took about 30 minutes (at room temperature).
The counter stain was performed in methyl green for 2 minutes at room temperature.
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