Sentence examples for minute and final from inspiring English sources

The cycling parameters for PCR included initial denaturation at 94°C for 5 minutes followed by 35 cycles of denaturation at 94°C for 15 sec, annealing at 55°C for 30 sec, extension at 68°C for 1 minute and final extension at 68°C for 15 minutes.

PCR was performed with a preliminary step at 95°C for 15 minutes followed by 35 cycles of 94°C for 1 minute, 62°C for 30 seconds, and 72°C for 1 minute and final step of extension at 72°C for 5 minutes.

The PCR conditions for DNA amplification consisted of 1 cycle at 95°C for 7 minutes; 35 cycles at 94°C for 30 s, 65°C (for M primer set) or 63°C (for U primer set) for 1 minute, 72°C for 1 minute; and final extension at 72°C for 10 minutes.

The PCR procedure consisted of an initial denaturation step at 94°C for 5 minutes and 35 cycles each of denaturation at 94°C for 1 minute, annealing at 56°C for 1 minute, extension at 72°C for 1 minute, and final extension at 72°C for 10 minutes.

The samples were denatured at 94°C for 3 minutes followed by 30 cycles of the following steps: denaturation at 94°C for 30 seconds, annealing at 55°C for 30 seconds, extension at 72°C for 1 minute, and final extension at 72°C for 5 minutes [ 6].

50 ng of genomic DNA from buffalo, cattle, goat and sheep were PCR amplified using reaction conditions 95°C for 5 minutes followed by 35 cycles each consisting 95°C for 1 minute, 60°C for 1 minute and 72°C for 1 minute, and final extension at 72°C for 10 minutes for all the four fragments.

We confirmed that bystander CPR, witnessed arrest, shockable initial rhythm, initial, average, 10-minute, 15-minute, 20-minute, maximum and final values of Pet CO2 and arrival time were all associated with improved ROSC and survival.

Situation-specific catastrophizing and pain ratings were obtained at Early (0 to 15 minutes), Mid (15 to 30 minutes), and Final (30 to 35 minutes) periods during capsaicin pain.

Documents in this series reflect the administration of the Studies Department (mainly through correspondence and subject files), the records of the groups themselves (through correspondence, background papers, meeting minutes and final reports), and the subject files and correspondence of major players in the Council's Studies Department from the 1940s onward.

When you start thinking of the real world in terms of quarterbacks, punting, first downs and marching on down the field, it is difficult not to think also in terms of half-times, closing minutes and final guns.

Thirty cycles of denaturation at 95°C for 1 minute, primer annealing at 58°C for 1 minute, extension at 70°C for 2 minutes, and final extension at 72°C for 10 minutes.