Sentence examples for minute and counted from inspiring English sources
All other segments were transferred to test tubes containing 1 ml saline and the adherent cells were dislodged by sonication in aquasonic device, vortexed for 1 minute and counted by trypan blue exclusion and viable count on Sabouraud Dextrose Agar.
It is noted that the pilot is actually two episodes, running together for 81 minutes and counted as only one (episode) by the creators and fans.
After 7 days, the spheres were collected by centrifugation (300 xg, 5 minutes) and counted.
Cells were stained with trypan blue (final concentration, 0.2%) for 5 minutes and counted using a hemacytometer.
Cells were then trypsinized, resuspended in 1×PBS and incubated in 2% trypan blue for 2 minutes and counted using a haemocytometer (n = 5 ± SD).
An aliquot of the HaCaT cell suspension was stained with 4% trypan blue for 1 to 2 minutes and counted with a hemocytometer.
We used a mercury thermometer placed in the axilla for five minutes and counted breaths over a 30 to 60 second period of settled behaviour[ 11].
Cells were trypsinized with 0.05% Trypsin-EDTA for 10 minutes, centrifuged at 900 rpm for 3 minutes and counted using the Trypan Blue assay on a Countess® automated cell counter (Life Technologies); only passages identified as greater than or equal to 95% viable were utilized in experiments.
In the reward and expectation scans we heightened their expectation of heroin reward by giving time checks every 10 minutes and counted down the last 30 seconds, and using a webcam so participants could see the injection, including the salient blood flashback.
Cells that remained in the upper chamber were removed with a cotton swab, and cells that had penetrated to the bottom side of the membrane were stained in Cell Stain Solution for 15 minutes, and counted in nine randomly selected microscopic fields (200×) per well.
