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However, to achieve such a pronounced LSPR shift when only a minute amount of sample material is available, or in a screening approach with many different recognition agents the volume has to be minimized and the accessibility of the analyte to the Au NPs has to be enhanced.
Multiplexed immunoassay can be performed in a capillary requiring only a minute amount of sample material.
Moreover, microfluidics is useful when only a minute amount of sample material is available for analysis.
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The diamond cell requires only minute amounts of sample material.
Our results demonstrate that a combined transcriptome and genome analysis is feasible from minute amounts of sample material.
This finding is in agreement with findings of other groups who analyzed minute amounts of sample material down to single cells [ 1, 11, 28, 33].
The benefit is twofold: First, it is possible to perform multiple experiments with minute amounts of sample and perform a statistical analysis of the data.
These technologies have the potential to deal with minute amounts of sample and, particularly in microfluidics, support multistep reactions and detection.
Recently, this system has been miniaturised to microchip format also known as microfluidic FFE (μ-FFE), making it attractive for the separation of minute amounts of sample coupled to very sensitive downstream MS techniques (Kohlheyer et al. 2008).
Enabling a profound transcriptome as well as genome analysis by next generation sequencing from minute amounts of sample material will have many applications and will enable the study of rare cells.
Recently, a stem-loop real-time PCR technology has been developed to determine microRNA expression levels, with a large dynamic range [17] in minute amounts of samples [18].
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