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This fragment connection algorithm provides "generic" 3D molecules in the sense that the linking part (minus the template) is built from an all-carbon chain whose synthesis may not be easily achieved.
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Remove mini bow from the template.
Consistent with the viral replication cycle whereby the plus strand genome is synthesized from a minus strand, mutations in the 5′-UTR decrease replication perhaps due to defective synthesis of the template minus strand (Yu et al., 2008).
In this case, the result is the emergence of a symmetry favoring the formation of either the plus-strand (replicase) or the minus strand (template).
Indeed, during minus-strand synthesis, when the template is RNA, resistance at M250 is more apparent than when the template for synthesis is the narrower DNA template of plus-strand synthesis.
Non-template controls containing all reagents minus the DNA template were included, to monitor the progress of template-independent ligation.
According to research by our group and Zeng et al., "surface imprinting" is more likely to be achieved during an inverse mini-emulsion polymerization process when the template molecules are amphiphilic.
Note, that a goodness-of-fit score represents the average z-score of voxels falling within the template minus the average z-score of voxels falling outside the template.
To do so, we used a "linear template-matching procedure" that involves taking the average z-score of voxels falling within the template minus the average z-score of voxels outside the template and selecting the component in which this difference (the goodness-of-fit) was the greatest [10], [20], [21], [22].
A second copy of this 11-nt sequence, called DR2, is located near the 5' end of the minus-strand template.
HP uses the viral pregenomic RNA (pgRNA) as the template to synthesize minus-strand viral DNA via its RNA-dependent DNA polymerization activity.
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