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Oligonucleotides complementary to 5′ and 3′ ends of the fragment encoding Kanr were used for initiating nucleotide sequencing from the plus and minus strands of pSP70, and thereafter primer walking was used to determine nucleotide sequence of pSP70.
These elements were found in both plus and minus strands of the chromosome and they showed a quite even distribution along the chromosome.
Although 1,633 Solexa sequences map to the plus strands of 147 VSPs, only 133 sequences map to the minus strands of 69 VSPs.
Since Conservation Plot does not consider strand information, sites belonging to the plus and minus strands of individual chromosomes were separated before conservation analysis, and then the minus strand results were reversed and averaged with the plus strand results.
The set of CRKs was divided into three groups (all, ozone up-regulated and ozone down-regulated) and the plus and minus strands of the promoters were searched for significant enrichment of single promoter elements or a combination of two promoter elements in either of the strands.
Analysis of our Solexa sequencing results reveal unequal frequencies of matching reads on the sense and antisense strands of Gireps, as shown in table 2. (i.e., the numbers of Solexa short-reads matching to the plus and minus strands of Gireps are uneven).
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Using this technique, two TALEN proteins must bind to the plus and minus strand of DNA to create a FokI heterodimer and form a double-stranded break.
These motifs were highly represented in the promoters of the plus or minus strand of the 12 defense-related genes.
Another example is CDR1as circRNA, which is derived from the minus strand of CDR1 gene and transcribed at opposite direction (Hansen et al., 2011).
Second, hairpins of antisense copies of the TE-MIRs located elsewhere in the genome may give rise to sRNAs that match minus strand of the corresponding TE-MIRs.
The dinucleotide composition of the minus strand of the HBV segment analysed is 20.5% TpC, 31.9% CpC, 25.0% GpC and 22.7% ApC.
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