Sentence examples for minus strain from inspiring English sources
If the cocktail is derived from E. coli, it is made from a Tus minus strain.
Thus, as with growth at the restrictive temperature of 30°C, after IR, the Dna2 nuclease minus strain is sensitive to damage, but only in the presence of an active Dna2 helicase.
When we tried to use a DNA fragment encoding pVT1_88 and encompassing the neighboring regions to confer autonomous replication to a suicide plasmid in a restriction minus strain of E. coli, no clones were recovered.
Although the analysis of the tra region suggests that conjugation is functional, initial attempts to transfer pVT1 through conjugation to E. coli after introduction of a suicide plasmid vector carrying a chloramphenicol cassette [41] in the gene encoding an IS4-like transposase (pVT1_36) were unsuccessful, even when we used a restriction minus strain such as DHα-T1 (see Material and Methods).
Chlamydomonas reinhardtii strains 21gr (mating type plus; CC-1690), 6145C (mating type minus; CC-1691), and the fusion-defective minus strain hap2 are available from the Chlamydomonas Genetics Center, University of Minnesota, St . Paul
Indeed in a recent primary study and accompanying meta-analysis, the increased gastric cancer risk due to infection by a Cag-minus strain compared to non-infection was four-fold [ 104], and the reviewed values in well controlled studies ranged from two-fold to five-fold.
C ag-minus strains are less infrequently associated with non-atrophic gastritis and duodenal ulceration [ 102].
Photoautotrophic growth would require steady-state ATP production, as has been demonstrated previously by growth deficiencies in BR-minus strains.
This is in contrast to the situation observed in macrophages infected with H37Rv, where PGL associated attenuation was only observed in pDIM-minus strains [ 56].
Electron microscopy with wild type, LLO minus and strain DP-L3078 was done twice with similar results.
Cyclaneusma minus strains were grown on 2% malt agar at 20 °C for approximately 14 21 days.
