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The reaction rates for plus and minus ends are different by a constant ratio δ [12], [13].
RNPs, microtubules, and minus ends are depicted as in A. t, time; d, distance.
In the bipolar spindle, this would mean that the minus ends are focused at the two poles.
The plus ends of spindle microtubules emanate into the cytoplasm to facilitate microtubule-mediated chromosome capture, whereas the minus ends are focussed at the two spindle poles formed by centrosomes.
In these cells centrosomes are present but organize fewer microtubules while most of the cellular microtubules are non-centrosomal and the minus ends are localized toward the plasma membrane without γ-tubulin anchorage.
Since we have identified a plus-end directed force (KLP-19) in the ring, and dynein inhibition causes chromosomes to be found further from the central pole, our data are most consistent with an organization in which the minus ends are in the center of the aster and the plus ends extend outwards.
Similar(46)
This observation is compatible with the notion that anchorage of RNPs at minus ends is facilitated by features that promote bidirectional movement.
Second, in new flagella, the ∼30-nm thick basal plate consisted of two stacked electron-dense rings, and the minus ends were found within the more distal ring.
It is intriguing that the average dwell time of pausing events of unidirectional hWT and hSL1x3 RNPs at minus ends is ∼ fivefold less than that of bidirectional RNPs harbouring the same RNA species.
Centrosomes organize symmetric microtubule arrays of uniform polarity, where microtubule-minus ends are embedded in the centrosome while the highly dynamic-plus ends extend toward the cell periphery.
Detachments of bidirectional hWT RNPs upon immediately reaching a minus end were rare (∼5% of total encounters).
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CEO of Professional Science Editing for Scientists @ prosciediting.com