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It's also worth noting that Accel Partner Jim Breyer's personal stake in Facebook is worth some $350,000,000 (minus dilution for subsequent venture rounds and employees since 2005) at this new valuation.
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A HORIBA MDLT-1300 partial-flow mini-dilution tunnel was used for PM measurement.
Titres were therefore reproducible on average with a range of plus or minus half a dilution step.
To detect the proximity between two proteins, complementary secondary probes (PLUS and MINUS) binding the primaries raised in different species were added to cells at a 1 5 dilution in blocking buffer for 60 min at 37 °C.
Relative ADCC activity was calculated by dividing the percent ADCC activity at each dilution minus background by the maximum percent ADCC activity measured across all dilutions.
Titers and the cumulative sum of optical densities of each dilution (SUMOD) minus the background absorbance of uninfected control Vero E6 cells (adjusted SUMOD) were recorded.
To detect primary antibodies with the in situ proximity ligation assay (PLA), the PLA probes mouse PLUS and rabbit MINUS (Abnova, UK) were added at a 1∶5 dilution in antibody dilution buffer (Olink Bioscience, Sweden) for 60 min at 37°C.
50 μg/well underwent electrophoresis on a 12% sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) mini gel (Pierce, Rockford, IL) after dilution in 1× Laemli buffer prior to denaturation at 95°C for 10 minutes.
We use the mini measure method: 5 μl of flow sample is added to 250 μl of diluted (1 in 5 dilution) Protein Assay Dye Reagent in a 96-wall plate.
A protein load of 50 μg/well underwent electrophoresis on a 12% sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) mini gel (Pierce, Rockford, IL) subsequent to dilution in 1× Laemli buffer with prior denaturation at 95°C for 10 minutes.
Log2 fold change was calculated as Log2-transformed fluorescence value of Gelsemium s. dilutions minus Log2-transformed fluorescence value of mean of controls.
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