Sentence examples for minus binding from inspiring English sources

To detect the proximity between two proteins, complementary secondary probes (PLUS and MINUS) binding the primaries raised in different species were added to cells at a 1 5 dilution in blocking buffer for 60 min at 37 °C.

Lysine-dependent plg binding was calculated as total plg binding minus binding in the presence of 1 mM tranexamic acid.

To further dissect lysine-dependent plg binding mechanisms in our experimental model, experiments were performed in the presence of the pln inhibitor aprotinin to allow a distinction to be made between pln-dependent binding (total binding minus binding in the presence of aprotinin) and pln-independent binding (residual binding in the presence of aprotinin).

Here, they find that it genetically interacts with and is synthetic lethal with mutations in Ptrn-1 (a minus end binding protein).

Since mini-agrin binds dystroglycan (rather that integrin α7β1), it is hypothesized that mini-agrin binding to dystroglycan is responsible for the restored regeneration [58], [59] and it has been demonstrated that dystroglycan activity in satellite cells is crucial for the maintenance of regeneration [10].

It has also been reported that the CKK domain (from Drosophila) binds uniformly along the MT surface, while minus-end binding requires the cooperation of multiple regions within the entire marshalin protein (Goodwin and Vale, 2010).

It ensures minus-end binding and stabilization of microtubules on the centrosome area that faces chromosomes.

This report examines the expression of marshalin, a minus-end binding protein, during this process of cochlear development.

Microtubule dynamics facilitate neurite growth and establish morphology, but the role of minus-end binding proteins in these processes is largely unexplored.

Thank you for sending your work entitled "The C. elegans microtubule minus-end binding homolog PTRN-1 stabilizes synapses and neurites" for consideration at eLife.

As an MT-minus-end binding protein carrying several protein-protein interacting domains, marshalin is likely involved in the establishment and maintenance of noncentrosomal MTOCs in SCs.