Sentence examples for mins to remove from inspiring English sources

Embryos were collected every 15 minutes and immersed in a 50% bleach solution for 2 3 mins to remove the chorion.

The liquid was then left at 37°C for 2 hr, cooled and centrifuged at 10,000× g for 30 mins to remove casein.

Harvested cells were suspended in 1/10th culture volume of Buffer A (−50 mM Tris, pH-7.5, 1 mM PMSF, 1 mM EDTA, 1 mM DTT, 10% glycerol) and lysed by sonication till O.D600 reached 1/10th the initial O.D. The homogenate was spun at 50,000× g for 30 mins to remove cell-debris.

The amylose resin was washed three times in buffer A. For total yeast cell lysate, Alp14p-13myc expressing mia1Δ cells were grown to log phase, harvested and washed once with Buffer A. Cell pellet were homogenized with glass beads (425 600 µm) using Mini Bead Beater (Biospec) at 4°C. Cell lysates were harvested and centrifuged (16,000 x g, 10 mins) to remove cell debris and insoluble protein.

Cells were freeze-thawed in lysis buffer and spun at 12,0000 g for 5 mins to remove cell debris.

The hybridisation cocktail was then transferred to 45°C for 5 mins and centrifuged at maximum speed for 5 mins to remove insoluble material.

The mixture was centrifuged at 8500 r.p.m. for 30 min to remove precipitates.

Before analysis, supernatants were spun at 300 × g for 5 min to remove floating debris.

Finally, the composites were degassed inside a vacuum for 20 min to remove entrapped bubbles.

Beads were pelleted at 300 × g for 4 min to remove supernatant.

Samples were centrifuged at 8,000 ×g for 10 min to remove any precipitates.