Sentence examples for mins to block from inspiring English sources

The balloon was inflated at 6 8 atm for 60 mins to block the blood flow completely without affecting the second diagonal branch.

The membranes were incubated with 5% (w/v) non-fat milk powder in TBS for at least 30 mins to block free protein binding sites.

Slides were then blocked with 1% bovine serum albumin (BSA) in PBS for 30 mins to block non-specific staining and then incubated with 4 μg/ml polyclonal rabbit anti-human E-Cadherin antibody (Santa Cruz Biotechnology, Santa Cruz, CA) in PBS for two hrs at room temperature.

And sequentially treated with 2.5% normal horse serum for 20 min to block nonspecific labeling.

Subsequently, the sections were immersed in 3% hydrogen peroxide for 10 min to block the endogenous peroxidase activity.

After centrifugation, the cells were resuspended in 5% donkey serum in 0.3% Triton-X for 30 min to block and permeabilize the cell membrane.

They were then treated with 0.3 % H2O2 for 15 min to block endogenous peroxidase.

Samples were incubated with 10%% goat serum for 30 min to block non-specific binding of the antibodies.

Ethanolamine (1M, pH8.5) was injected for 7 min to block the remaining activated groups.

An excess amount of sulfo-NHS acetate was introduced and kept for 15 min to block the unreacted amine groups.

The cells are left at RT for 10 min to block aspecific sites on the cell surface.