Sentence examples for mins in order from inspiring English sources

After removal of Accutase and gentle trituration in passaging medium (complete culture medium containing 20 ng/ml basic FGF and 10 μM Y27632 (Stemgent), cell suspension was pre-plated in gelatin (0.1% in PBS -coated 100 mm dishes twice for 45 mins in order to remove residual MEF feeder cells.

Cells were then enumerated by flow cytometry with a CyFlow®space instrument (Partec, Canterbury, UK) using side scatter and fluorescence when stained with 5 µM SytoBC (Molecular probes, Invitrogen, Paisley, UK) for 10 mins in order to get a monodispersed suspension free of large flocs or planktonic micro-colonies, ensuring an accurate serial dilution of the inoculum for spiking.

A CXCR4- and MMP9-expressing cell line (U87.CD4.CXCR4) 3 was incubated in the presence of the NPs for different periods of time (15, 30 and 60 mins) in order to find an optimal incubation time.

Mice were placed inside the free-moving plethysmograph chamber and allowed to acclimate for 5 min in order to minimize any effects of stress-related changes in ventilation.

Thereafter, methanolic HCl was added and samples were heated up to 85 °C for 45 min in order to synthetize methyl esters.

The time of deposition was varied between 1 and 10 min in order to obtain different thickness of vanadium films.

The substrates were immersed in PAA or PDDA solution for 10 min, followed by washing in DI water for 10 min in order to assemble two layers of PDDA and PAA.

200 μL of cultures were spun down (11000 g, 5 min) in order to remove the culture medium; and the obtained pellets were suspended in 200 μL of 5% Chelex 100 (Bio-Rad) with 0.2 mg protease K.

Then, the tube was heated in a water bath at 75 °C for 5 min in order to evaporate the main portion of the extraction solvents.

The subjects then performed intermittent typing workload for 30 min in order to induce acute shoulder stiffness.

Finally, the pastes were vibrated for 5 min in order to release bubbles.