Sentence examples for mins at room from inspiring English sources

Brain slices were fixed in 4% PFA at 4 °C o/n with shaking and washed in PBS for 30 mins at room temperature (RT).

Following incubation, cells were fixed using 4% paraformaldehyde in PBS for 15 mins at room temperature followed by rinsing and washing with ice-cold PBS twice.

To compare living biomass with dead cell beads by image analysis, we initiated cell death deliberately induced as negative control in beads by exposure to 50% (w/v) Me2SO for 5, 10 and 15 mins at room temperature, which we have established, produces a controllable lethal insult.

After rinsing with PBS, sections were incubated in 3% BSA for 30 mins at room temperature.

Lipoplexes were then combined and further incubated for 15 mins at room temperature.

The other cultures were fixed in ice-cold methanol for 15 mins at room temperature prior to processing for immunohistochemistry.

Briefly, cells in three 150 mm plates per condition were crosslinked with 1% formaldehyde for 10 mins at room temperature.

Control and treated cells were washed with PBS and fixed in 1% paraformaldehyde (15 mins at room temperature).

Inner membrane proteins were pre-extracted by 0.5% sarkosyl in 10 mM Hepes/50 mM NaCl pH 7.5 for 30 mins at room temperature, followed by centrifugation.

After 30 mins at room temperature, 30,000 counts per minute (cpm) of radiolabelled oligonucleotide probe was added and incubation continued for an additional 30 mins.

The cells were then washed in borate buffer (0.1 M, 12 mins at room temperature) and incubated with sheep anti-BrdU (Abcam; 1∶500) overnight.