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Compounds were eluted over 33 mins at a flow rate of 3 mL/min with methanol/water (9∶1) with linear ramping to 100% methanol.
Vehicle (1% N-lauroyl sarcosine for 2A perfusions and 0.9% NaCl for 5-biotinamidopentylamine (5-BP) perfusions) was perfused through 20 cm of the rat jejunum for 15 mins at a flow rate of 24 ml/hr.
The NHS-carboxy groups of spot 1 of the dextran surface were treated with a solution of neutravidin (50 µg/ml) in sodium acetate (pH 5.0) for 8 mins at a flow rate of 10 µl/min.
The NHS-carboxy groups of the dextran surface were treated with a solution of AKT1 or GRP58 (25 µg/ml) in sodium acetate (pH 5.0) for 8 mins at a flow rate of 10 µl/min.
The UV-C treatment was achieved by exposing the abaxial surface of the discs to 30 W UV-C light for 10 mins at a distance of 10 cm.
Separation was achieved using a 45-minute linear gradient of 10 to 45% solvent B (90%CH3CN/0.1%% formic acid) versus solvent A (2% CH3CN/0.1% formic acid), then to 90% B for an additional 5 mins, at a flow rate of 300 nl/min.
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Mosquitoes are fed by AF for 15 min at a time.
Depositions were carried out for 45 90 min at a potential of −1050 mV vs. sce (45 °C).
The run time was 8 min at a flow rate was 1 mL/min.
All samples were centrifuged at 2,300 rpm for 15 min at a temperature of 4 °C.
The media was autoclaved for 15 min at a pressure of 1.05 kg/cm2 at 121°C.
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