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Evaluation of the initial contigs by homology comparisons of the sequences with themselves indicated under-contigging, with highly similar sequences probably remaining from allelic variants (from heterozygous source DNA), splice variants, and minor sequencing variants.
Minor sequencing errors could produce different theoretical proteomic profiles, affecting the correctness of the PSMs identified.
Rare tags could originate from infrequently expressed transcripts but could also reflect minor genomic contamination or minor sequencing errors.
Results showed that the first 16 bases showed minor sequencing bias so these were trimmed from the sequencing reads.
Moreover, minor sequencing errors would have little or no effect on the genome comparison by MAUVE which examines large homologous blocks between strains.
Less stringent alignment parameters were used for this pass to allow for minor sequencing errors or allelic differences in the cDNA sequence.
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Incorporation of SILAC helps to distinguish minor sequence variations between original and deduced sequences.
The microstructural analysis of these copolymers suggests that these are alternating copolymers with meso (major) and racemic (minor) sequences.
Our data suggest that FabH utilizes protein dynamics and seemingly minor sequence and structural differences to modulate its molecular recognition and substrate specificity across bacterial species.
The pairwise distance calculation inside the P. bucharensis group showed that the collected P. bucharensis species were identical with a minor sequence variation to strain CU_7.
This might be due to the wide distribution of V. orientalis in Egypt, Jordan, and Israel, where the social wasp fauna (Vespinae, Polistinae) in Jordan is similar to faunas of Israel and Egypt (Zalat 1992; Haddad et al. 2007) while strains isolated from El-Fayoum Governorate, CU_5 and CU_6, had minor sequence variation from the other collected species (Table 2, A).
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