A total of 32 sequence polymorphisms (minor allele frequency MAF >/= 1%) were identified in 96 healthy control samples (12 in the putative promoter region, 2 in the coding region, 16 in the introns and 2 in the 3' UTR).
After linkage analysis, 9 of the 13 polymorphic loci of pre-miRNAs were mapped on 7 cotton chromosomes (Chr01, Chr04, Chr13, Chr15, Chr16, Chr23 and Chr26) based on SSCP analysis which can discover minor sequence mutations and reveal more polymorphisms to map genes [ 35].
Thirty of the most consistently amplifiable gene fragments, both within Vitis and outgroups, with suitable polymorphisms and only minor sequence length variation in the eight tested, were re-sequenced in a total of 309 accessions (Additional file 1).
Only sequence polymorphisms with less than 20% missing data and with a 0.2 minor allele frequency were analysed.
Although many of the 11,941 SNPs with a minor allele count equal to one may be true SNPs, this class is undoubtedly enriched for erroneously identified sequence polymorphisms.
Although developed for analysing experimentally induced minor sequence differences, our procedures, PAGE in particular, will find a wide variety of applications in detection, quantification and enrichment of polymorphisms and alterations in basic and applied research as well as medicine.
We evaluated sequence polymorphisms of PbP27 gene sequence among isolates, finding some polymorphisms associated with the isolates' phylogenetic origin.
Incorporation of SILAC helps to distinguish minor sequence variations between original and deduced sequences.
Without evaluation data, for example, it is not possible to link sequence polymorphisms with phenotypic performance.
