Indeed, functional flexibility of acyl-CoA carboxylases may be of major importance in relatively rapid adaptation strategies concerning bacterial metabolism [ 5, 54, 55] since minor precise sequence modifications have been shown to easily change their function [ 33].
Illumina small RNA sequencing protocol was used for sequencing with minor modifications as previously described [ 18].
RNAs corresponding from 15-50nt frelative relatove to ladder were excised for from the PAGE gel and purified for sequencing using the Illumina small RNA sequencing protocol with minor modifications as previously described [ 44].
The protocol was developed for the Illumina platform, but most of the steps are adaptable to other sequencing platforms with minor modifications.
Since TargetScan (version 6.0, [ 51]) does not support zebra finch sequences, we made minor modifications to adapt it for target analysis in the zebra finch.
Because the long march relies only on minor modifications to adapter sequence and an appropriate Type IIS or Type III restriction enzyme, it can be readily customized for a variety of applications.
Although designed for use on humans, this protocol could also – with minor modifications – be used to sequence small, organellar genomes from other species, and also nuclear loci.
To successfully transfer the scaffold design to ITS2 sequencing, we ensured by minor modifications that the melting temperature (Tm) of the combined pad, linker and amplifying primer was ~65°C (see Additional file of Kozich et al. [ 16]) enabling the read primers to bind during the later sequencing procedure.
Several minor survey modifications were made prior to the launch.
To generate finite memory transmit sequences, a minor modification of the presented state machine is required, which is described in Appendix B. This modification leads, depending on the source memory Lsrc, to a slight reduction of the source entropy rate.
