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More typically, clones not resulting in detectable GFP expression had minor sequence errors including amino acid substitutions.
We used PHRED score 15 as cutoff value for quality sequences because the most meaningful annotation analysis is BLASTX that has high tolerance to minor sequence errors.
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Rare tags could originate from infrequently expressed transcripts but could also reflect minor genomic contamination or minor sequencing errors.
Less stringent alignment parameters were used for this pass to allow for minor sequencing errors or allelic differences in the cDNA sequence.
Moreover, minor sequencing errors would have little or no effect on the genome comparison by MAUVE which examines large homologous blocks between strains.
Regular expression is adopted for customized filtering requirements, for instance, allowing read mismatches to tolerate minor sequencing errors, extending match length for extra 'identifier sequence' to exclude misprimed reads, and preloading endogenous elements from a configuration file to avoid them.
The mt genome of Xenopus laevis was the first ever determined for an amphibian [ 34] and contained numerous minor sequencing errors probably due to technical constraints at that time.
Similarly, in contigs with 4 sequences when the minor sequence allele is represented only once, it is highly likely that the minor allele is due to sequencing errors.
These errors often occurred at two ends of a homopolymer run due to high coverage of the original reads; for example, a sequence "AAAAAAAAAC" often displays a minor genotype for C in position 10, but this C is often an artifact due to sequence errors intrinsic to pyrosequencing.
Therefore, setting a minimum minor allele frequency (e.g. 10%) for larger contigs may help reduce false SNP calling based on sequence errors.
We searched for unique kmers found only in male or female fish, with a minor allowance for sequencing errors (max two individuals).
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