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Moreover, phylogenetic analyses under the ML framework using specific substitution models for different partitions of the data most likely accounted for any minor sequence bias that could have affected the analyses [ 31, 50, 51, 54, 59].
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Results showed that the first 16 bases showed minor sequencing bias so these were trimmed from the sequencing reads.
Analysis of the TILLING data revealed that EMS is a nearly ideal mutagen, producing G/C-to-A/T transitiofs >99% of the time with only minor local sequence biases [ 17].
Incorporation of SILAC helps to distinguish minor sequence variations between original and deduced sequences.
However, a clear sequence bias is evident.
we run the risk of incorrectly adjusting for biological sequence bias, rather than technical sequence bias.
Similarly, in contigs with 4 sequences when the minor sequence allele is represented only once, it is highly likely that the minor allele is due to sequencing errors.
The microstructural analysis of these copolymers suggests that these are alternating copolymers with meso (major) and racemic (minor) sequences.
Rare tags could originate from infrequently expressed transcripts but could also reflect minor genomic contamination or minor sequencing errors.
In addition, technical variance, sequencing bias and mapping bias have been reported [ 9- 11].
Clearly Sfd = m y if sequencing bias is free.
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