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While products of nature are currently excluded, even minor modification that introduces the elements noted above may allow patenting to proceed.
Immunoprecipitation experiments were conducted essentially as described [20], with the minor modification that all lysis and wash buffers contained 20 mM MgCl2.
This was performed as described (Teismann et al., 2003) with the minor modification that incubation with primary antibodies was performed sequentially as detailed below.
Plasma vitamin A was measured by reverse-phase high-performance liquid chromatography by the method of Thurnham et al. (1988) with the minor modification that hexane was used for extraction instead of heptane.
E. coli serotyping was conducted following a protocol previously described [ 28], with the minor modification that the multiplex PCR assay was carried out on DNA extracted from a smear of bacteria from the first area of a MacConkey plate.
RNA extraction from biopsies was performed either with the Ultraspec-II RNA isolation system (Biotecx, Houston, TX, USA) according to the manufacturer's protocol or with the Qiagen RNeasy minikit (Valencia, CA, USA) with a minor modification, that is, the amount of lysis buffer was increased to 500 μl.
Similar(52)
The detailed RSVP-TE state recovery generally follows RSVP-TE GR with minor modifications that can be performed in the background in parallel with normal RSVP-TE operations for connection setup and removal.
With p154 but few minor modifications that plan was accepted by the Military Committees of both houses of Congress and became a law.
Quon said markers now reflect a 2006 mapping survey of the vernal pools and any minor modifications that could be ascertained considering the dry conditions during the team's visit.
Several minor modifications that resulted from further development of the model were introduced.
Rather, we aimed to use an existing methodology with minor modifications that were required in our particular case.
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