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If this is indeed the case, Pae has found a new host, European horse chestnut, on which it is highly mobile and aggressive, causing frequently lethal stem cankers (Figure 1A) that contrast with the minor leaf lesions observed on Indian horse chestnut (Figure 1B).
On the other hand, T. populina induces less deformity than others; this fungus infects Populus trees and causes only minor leaf spots (Rodrigues and Fonseca 2003).
However, besides a minor proportion of simple nitrile formed in T. minor leaf and flower homogenates, there is no indication for the presence of specifier proteins with a function in glucosinolate breakdown outside the Brassicaceae.
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Using this classification system, we qualitatively surveyed the abundance of wall ingrowth deposition in PP TCs in terminating minor veins across leaf development, selecting leaf 11, 8 and 5 as representative of immature, intermediate and mature leaves, respectively.
Tissue samples were collected along the minor veins near leaf apex, then fixed for 30 minutes at room temperature in a 0.1 M phosphate buffer (pH 7.0) containing 1% glutaraldehyde, and embedded in 7% low melting-point agarose and sectioned (100 µm thick) transversally with a razor blade using a vibratome (Series 1500 Leica St . Louis MO).
Additional file 1: Movie S1. z -stack scan of Arabidopsis leaf minor vein showing wall ingrowth deposition in PP TCs.
In addition, a minor QTL for leaf area was found on chromosome 4 of the same parent.
Additional file 3: Movie S3. z -stack scan of Arabidopsis leaf minor vein from fixed tissue extracted with bleach.
This method was used to image wall ingrowth deposition in PP TCs in rosette leaves, cauline leaves, cotyledons and sepals of Arabidopsis as well as CC TCs in leaf minor veins of pea.
To test the general applicability of this method for imaging wall ingrowths in other species, we used the mPS-PI procedure of Wuyts et al. [ 17] to stain CC TCs in leaf minor veins of pea, where reticulate wall ingrowth deposition occurs on all faces of these cells [ 19, 20, 24].
This location coincides with the location of mapped qBlsr3a, a minor QTL for bacterial leaf streak resistance in rice Acc8558 (Tang et al. 2000).
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