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With the MiSeq™ (Illumina) three fresh-frozen samples were analysed and minor frequencies of the mutated allele could be detected.
As the frequencies of the alleles play an important role in LD analysis, we eliminated the alleles with minor frequencies of less than 0.05.
Both were identified in the 16S rRNA gene amplicon dataset, as well as in the whole metagenome approach, in the two biogas plants with minor frequencies.
In Saudi Arabia all of them have representatives albeit in minor frequencies, K (4%) and U3 (2.3%) being the most abundant clades.
The frequency "0" and the first quartile [0 0.25] represent 15 loci in a category of segments lacking or with ancestral haplotypes at minor frequencies.
With the GS Junior (Roche) no minor frequencies of mutated alleles were seen at four positions of secondary mutations (p.V654A, p.N680K, p.D820E, p.N822Y).
Similar(53)
SNP alleles forming the minor frequency haplotype are italics.
All minor frequency SNP alleles increased average TSC and TSY (Table 3).
Effects are reported for the minor frequency allele (MFA).
Among participants with at least one minor frequency allele on Bsm1, 98% had at least one minor frequency allele on Taq1.
Frequencies are given for truncations and for the minor frequency haplogroups.
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