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The use of so few patients also makes it difficult to separate any clinically relevant results from those attributable to the patient idiosyncrasies, and is likely to induce greater change in the minor fixation findings.
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In his case, it was musings on foreign policy, observations about the cognitive abilities of his colleagues and a not-so-minor fixation on the appearance of women, generally expressed ungenerously.
Staining with anti-p-Smad1/5/8 antibody (1 200, Cell Signaling) was performed with minor modifications: fixation in 2% PFA in PBS for 1.5 hours at RT; blocking in 10% goat serum/0.3% Triton X-100 in PBS.
Although these previous studies showed that there are minor differences in fixation patterns between certain groups, the sequence of fixation on the eyes and mouth has been considered to be a universal information extraction pattern.
Typically, the implant is rigidly applied to the underlying bone, making appropriate lateral translation of the distal fragment and minor adjustments after fixation very difficult.
Based on this and previous fMRI studies (e.g., Tootell et al. 1998), such foveal V1/V2 patches likely reflect a minor variability in fixation stability across conditions.
The latter is almost claustrophobic in its fixation on minor details and export control restraints.
I have only a minor point concerning the fixation and maintenance of duplicates.
A total of 925 of the routine cytology samples were reviewed by an independent expert, who noted at least minor deficiencies of sample fixation, staining or mounting in 19.8% of all slides.
In addition, segregating hypomorphic alleles are rare on the X and thus play a very minor role in reducing fixation probabilities on the Y (supplementary fig. S9, Supplementary Material online).
On a worldwide scale, however, these alternate arrangements of nitrogen fixation are relatively minor compared with those supported by legumes.
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