In contrast, only minor expression was found in the MDH of control animals that received aCSF without strychnine before stimulation (Figure 5A).
TIMP-4 transcripts were predominantly localized to tumour cells, though minor expression was found in vessels.
Interestingly, the U6atac increase correlated with enhanced minor intron splicing and gene expression of a splicing reporter (LucI-minor) whose expression is strictly dependent on splicing of a minor intron, showing a twofold increase of its expression after anisomycin treatment.
Accumulation of starch and minor RBR expression were characteristic of megagametophyte cells in the ESR, whereas strong RBR expression was found in the cells of the nucellar layers at the late embryogeny.
In practice, constant expression of those transcripts is not a requisite in the invasion model as long as the variation of the expression is minor relative to the variation of the lymphocyte cell abundance due to the infiltration, according to the equation (5) in Additional file 2: Supplementary Methods.
This increase was completely blocked after co-treatment with U6atac AMO, even at a low amount (1 nmole) that only partially inactivates U6atac, confirming that p38MAPK up-regulation of minor intron-containing mRNAs expression is strictly reliant on its ability to increase U6atac level.
This observation generalizes a previously reported specific case for OR51E2, which was found to be over-expressed in human prostate [ 39, 40], although minor expression could be detected also in brain.
However, because of its inconsistency in binding to AIE RNA probes, a possible role of this protein in age-related regulation of gene expression is likely minor, if any.
Although this is a minor liver P450, its polymorphic expression is associated with the abnormal metabolism of at least 30 therapeutic drugs, including beta-blockers and tricyclic antidepressants.
With this in mind, and given the length of our transcription experiment, it is likely that even these 2 windows of minor expression may be the result of something other than actual induction of the sigS operon (e.g. cellular lysis).
