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In the CRF02_AG phylogeny we identified one major and four minor clusters of Guinea-Bissau-specific sequences, indicating a scenario of multiple introductions into Guinea-Bissau (Figure 1a).
Cartilage-treated specimens were observed to present the occurring of minor clusters of cell dispersing in the OA articular cartilage.
Two of the subgroups from the ADMIXTURE analysis were not wholly supported by PCA, however, as some of the genotypes from these two minor clusters of ADMIXTURE were mixed with the major PCA groups.
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We accounted for this minor clustering of the full cohort data by utilising a sandwich estimator (the cluster command within Mplus, combined with the complex samples approach).
Another set of primers (napin-related) was designed specifically for the minor cluster of B. napus assembled EST clusters grouping in-between the four A. thaliana 2S seed storage proteins and the related protease inhibitor/seed storage/lipid transfer (LTP) family protein AT5G54740 (about 47%to57%7% identity on the aa level).
To reflect some complexity we added minor clusters to some of the clusters.
Besides clusters of tandem repeats, a number of minor clusters related mostly to Gypsy and Copia LTR-retrotransposons occurred (fig. 1).
A PCA of Guinea-Bissau ethnic groups is illustrated in Figure 4b, less biased by the major influence of haplogroup E3a*-M2 and where the influence of minor clusters is emphasized.
We observed that the majority of structural models (177/265) are grouped in a single cluster while other two minor clusters contain the rest of the models (47/265 and 41/265).
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