Although in this experiment, starting with less than 1 μg of total RNA did not produce enough biotinylated cRNA for hybridization under the normal protocol, a minor change (using vacuum evaporation to concentrate samples before cRNA synthesis, and mixing the minimum 200 μl hybridization volume) should allow use of 1 μg samples with the standard protocol.
The original North American and Japanese NTSC versions and the second North American version, which contained minor changes, all used a loader that sometimes caused the game to crash in specific rooms.
We performed Western blotting and ELISA analysis as previously described (Detweiler et al. 2002) with minor changes (we used fish gelatin instead of casein to prevent the nonspecific binding sites).
RNAPII, p63, H3K27ac, RUNX1, and TFAP2 ChIP experiments were performed as previously described [ 65], with a minor change of using magnetic beads (Novex by Life Technologies, 10008D and 10009D).
"They only had to make minor changes to keep using it successfully," noted Del Rosso.
In our experiments, we observed minor changes, since we used only squalene concentrations up to 0.4 mol%.
With some minor changes, the boundaries used for the health regions correspond to the geography of the 1996 Census.
The specified algorithm with some minor changes can be used to compute the minimum free energy joint structure of two interacting nucleic acid strands.
Based on this, a certain amount of horizontal scaling can be achieved with minor changes, simply by using commodity hardware for additional download servers and requiring only the GT server software to be installed.
RNAs were processed as described previously with minor changes [60] for use on Affymetrix GeneChips Mouse Genome 430 2.0 (Affymetrix, Santa Clara, CA, USA) and analyses were done with dChip Software.
