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When grown at 22°C, cells displayed their characteristic elongated, cigar-shape morphology although frequently they were thicker than wild-type cells and showed minor bud neck defects (i.e. 32% of the cells had bendy or wider bud necks, n = 100 cells; Fig. 3C).
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It is difficult to detect minor increases in DNA content by flow cytometry, however, and small buds are difficult to detect microscopically.
In mid-November, both cultivars at ambient temperature and 'Narve Viking' at elevated temperature were endo-dormant, with only minor signs of bud development after lasting forcing.
For example, Mus81- Mms4 plays a major role in fission yeast (Smith et al. 2003), but only a minor role in budding yeast, Arabidopsis, mouse, and Drosophila (De Los Santos et al. 2003; Argueso et al. 2004; Berchowitz et al. 2007; Trowbridge et al. 2007; Higgins et al. 2008; Holloway et al. 2008; Jessop and Lichten 2008; Oh et al. 2008).
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For example, in situ hybridization and microarray analysis shows that Sox17 is highly expressed in bladder stroma and to a minor degree in the ureteric bud and in metanephric mesenchyme at E11.5 days after fertilization, a stage where Wnt9b acts to regulate ureteric branching [Carroll et al., 2005].
"A little bit of powder on the taste buds gives you all the flavor of a lot of chocolate mass that you chew on and break down in your mouth to accomplish the same purpose," he said, adding that when you eat chocolate, "the actual percentage of that chocolate that stimulates your taste buds is very minor".
The matrix protein (VP40) is required for budding of the virus particle from the plasma membrane; the minor matrix protein (VP24) facilitates virus budding [ 8].
During the 1980s, he grabbed national headlines by creating a "unicorn," attaching a horn bud onto a goat with minor surgery.
During the 1980s, he grabbed national headlines by creating a "unicorn," grafting a horn bud onto a goat with minor surgery.
Therefore, one can expect a complex genetic control of bud formation by many genes with minor effect on the phenotype.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com