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After two hours of incubation, minor bands below the large product were evident but these represented less than 5% of the total products produced (data not shown).
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The minor band migrating below HA-tagged eEF1A lacking domain II likely represents a proteolytic degradation product of the protein construct.
The minor band visible below the major SSI band in the double mutant also requires a functional AtSS1 gene (data not shown) and thus is identified as a form of that enzyme.
Some minor bands were seen though.
Additionally, one band for U6ab and three minor bands for U6us can also be seen.
The normal M variant yields a characteristic five-band pattern: two major bands and three minor bands.
The nasal mucosal extract also had major bands with pI 4.8-5.2 and several minor bands with pI of 5.3 - 6.2.
Lhcb1 was detected as one major band in pea and one major and two minor bands in bean samples.
SDS-PAGE of the unfertilized egg envelope yielded a major 44 kDa band and several minor bands.
VML migrated as four bands, two major bands (double bands) of approximately 34 and 32 kDa (α-chains) and two minor bands of 22 and 13 kDa (the β- and γ-chains, resp .. LAA migrated as two major bands of 29 kDa (double bands) and two minor bands at approximately 15 kDa.
Furthermore, although both seasons had many minor bands, few of them existed in both seasons.
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