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The MinION will read both strands of DNA (Template and Complement) and the software will attempt to call a consensus 2D (2-directional) read.
The MinION flowcell (R7.3 flowcell chemistry) was run for 24 hours using the MinKNOW software (v47.3) producing 24,859 fast5 files, corresponding to individual reads from base detection events at specific nanopore channels.
Users of the MinION must buy a high-specification Windows laptop, and thus there is a need for Windows-based software to handle the data.
The most fundamental functionality provided by poretools is the ability to convert the output data resulting from a MinION run from HDF5/FAST5 format to either FASTA or FASTQ format to facilitate analyses with sequence alignment and/or assembly software.
Minion merchandise is rampant.
Too garrulous minion, stop.
"I'll be a minion.
A minion appears.
MINION: Genius counter-programming.
All hail Minion Yellow.
MINION: But not too urban.
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