Sentence examples for mining the unigenes from inspiring English sources

Second, intraspecific EST-SNPs were developed by mining the unigenes of G. hirsutum.

By mining the tomato Unigene and BAC sequence databases from the Sol Genomics Network with homology searches and subsequent sequence extension by RACE, we initially identified and cloned 24 different tomato genes encoding putative TCP transcription factors (Table  1; SlTCP1-24).

We therefore mined the EUCAWOOD unigene set for genes involved in lignin biosynthesis, carbohydrate and cell wall metabolism.

By mining the EST database, we found 125 unigenes (500 ESTs) annotated as putative CYP genes, which were further classified into 32 CYP families and 47 subfamilies (Additional file 4).

To fill the gap in knowledge about sequence similarities and differences among cultivated and wild tomato species, we mined the EST and unigene tomato database from SGN in search for substitutions and insertions/deletions between and within the three species with highest representation: S. lycopersicum, S. habrochaites and S. pennellii.

We mined the Arabidopsis, grape, poplar, Medicago and soybean sequence databases to identify genes paralogous and orthologous to the 31,798 Sanger-based oak unigenes described by Ueno et al. [ 33].

Junaid Iqbal mined the literature.

2 HAU-SNP357 ~ 811, which were developed by mining G. hirsutum unigenes.

A total of 1,349 EST-based SNP and InDel markers were developed by comparing ESTs between Gossypium hirsutum and G. barbadense, mining G. hirsutum unigenes, and analyzing 3′ untranslated region (3′UTR) sequences.

The unigenes were mined for microsatellite motifs.

We validated the in silico mining method by aligning EST mined unigenes with four full length cDNA clones of grass IRI-like genes from the NCBI core nucleotide collection (barley; AK252915, AK249041/wheat; AY968589, AY968589).