Sentence examples for minimum two generations from inspiring English sources

For retinal depletion experiments, flies were reared for minimum two generations on carotenoid-free medium (10% yeast, 10% sucrose, 0.02% cholesterol, 2% agar) [57].

Their descendents were reared in freshwater for a minimum two generations.

Some effects of metabolic disruptors may be transgenerational, requiring a multigenerational approach: a minimum of two generations for paternal line effects and three generations for maternal line effects.

The families all consisted of individuals from a minimum of two generations (parent and child), with the largest family encompassing four generations.

Eight mouse strains with distinct mutations in Col4a1 and one mouse strain with a mutation in Col4a2 (25, 43) were backcrossed to C57BL/6J mice for a minimum of five generations to generate an allelic series of Col4a1 and Col4a2 mutant mice on a uniform genetic background (Supplementary Material, Table S1).

To circumvent this problem, genetically modified strains have generally been backcrossed for a minimum of eight generations onto the DBA/1 background, which introduces a delay of 1 to 2 years.

Heterozygous mice were backcrossed a minimum of six generations with C57BL/6 mice prior to the study.

Mice harboring the conditional Actg1 allele were described previously [28] and subsequently backcrossed a minimum of five generations to the C57BL/6 background.

Four replicates of both the infected and uninfected flies were reared on Lewis medium [21] at a constant density of approximately 220 flies per bottle at 25°C on a 12 hour light-dark cycle for a minimum of three generations before the experiment.

Wild-type lines were subjected to single-pair sibling mating for a minimum of nine generations.

The mice used for analysis were backcrossed to C57Bl6 for a minimum of four generations.