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Based on the above data, we propose minimum transcript level 50 (MTL50) as a measure of stringent requirement, somewhat similar to the IC50 measure of enzyme inhibition.
The relationship between mRNA decline and growth rate decline reflects the degree of essentiality, or stringency, of an essential gene, which is here defined by the minimum transcript level for a 50% reduction in growth rate (MTL50).
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Using a signal intensity cutoff of 200 as the minimum, transcript levels in heterocysts plus the phosphoserine phosphatase activity detected in the crude extracts of heterocysts of phototrophic cultures (footnote f of Figure 5) suggest that Gln, Glu, Ser, Gly, Cys, Thr, and Pro are actively produced in heterocysts.
The deduced amino acid sequences of the three OMTs showed high sequence similarity (Additional file 5) with a minimum pairwise identity score of 90% at the genomic level and of 96% at the transcript level.
Fgf-2 transcript was down-regulated, while Fgf-18 transcript level was strongly up-regulated.
Y-axis, relative transcript level compared with rice OsUbi1.
Interestingly, EGTA treatment repressed the transcript level of OsNCX genes.
Y-axis, relative transcript level compared with OsUbi1.
Transcript level of each gene was normalized to the transcript level of rice TF2E (Os10gene7200) gene (used as a control gene).
The expression of PhaPR1 transcripts was reduced when the transcript level of PhaNPR1 was down-regulated (Figure 5).
At the transcript level, OsCDPK13 responds to various abiotic stresses as well as to hormone levels.
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