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Sera from healthy controls exhibit minimum titer of Ma2 autoantibodies, determined by the indirect ELISA and shown by the low recognition of GST-tagged PNMA2 recombinant protein (lanes III and IV).

For both wild-type HSV-2 and HSV-2 ICP0− mutant viruses, viral stocks were generated for animal experiments that were concentrated 10-fold by ultracentrifugation to achieve a minimum titer of 3×107 pfu/ml.

For both wild-type HSV-2 and HSV-2 ICP0− mutant viruses, viral stocks were generated that were concentrated 10-fold by ultracentrifugation to achieve a minimum titer of 3×107 pfu/ml.

This suggests that the minimum titer of baculovirus for GFP fluorescence can still induce cytotoxic effects sufficient to cause a pupal death.

In this assay, a minimum titer of SCSMV could be determined for as low as 100 copies of ssRNA or 2 pg total RNA.

Similar(55)

Serum HI titers in both groups were increased following the first boost to levels that clearly exceeded the minimum titer associated with protection in humans (titer >40 HI).

ELISA responders were defined as participants with a minimum titer ≥1∶500 and a >2 fold increase after immunization.

The possibility also exists that a minimum detectable titer of 1 10 as the threshold for seropositivity may not be indicative of true immunity.

Although DENV NAbs are widely considered to be a potential primary correlate of protection against dengue disease, 7 no protective threshold titer similar to the minimum protective titer of 1 10 for Japanese encephalitis virus has yet been established for DENV.

The secondary objective was to calculate minimum titers required to make diagnosis of typhoid and paratyphoid fever in Nepal.

For calculation, negative titers were assigned the value of half the minimum detectable titer, and titers greater than the final dilution were assigned a value 2× the largest titer.

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