Sentence examples for minimum tiling paths from inspiring English sources
The minimum tiling paths of contigs I, II, and III are 15, 4, and 4 BAC clones, spanning approximately 1900, 400, and 300 kb, respectively.
The genetically defined borders coincide with the common BACs on the minimum tiling paths of HSY and X.
This affirmed that end sequence was produced from the majority of the BACs in each pool, and that the coverage across the minimum tiling paths were high.
The minimum tiling paths of HSY and its X counterpart are being used for sequencing these X and Yh-specific regions.
The BAC library described here will be valuable for accurate assembly of the porcine genome sequences, the physical mapping of the pig genome, and the construction of minimum tiling paths for region-specific resequencing.
The utility of BAC clones as substrates for end sequencing, in conjunction with advanced DNA techniques and microarray analysis, has permitted construction of robust physical maps and selection of BAC minimum tiling paths.
Nine BACs were required to span the contig in a minimum tiling path (Fig. 1); eight tiled BACs were selected for GS FLX pyrosequencing and the final (ninth) BAC was sequenced using standard Sanger sequencing of a shotgun library.
The remaining 73,070 BACs were selected from a minimum tiling path representing the physical map of the barley genome16.
This comprised 15,661 so-called gene-bearing BAC clones, preselected mainly by overgo-probe hybridization for the presence of transcribed genes and fingerprinted for definition of a minimum tiling path of the barley gene space.
These gene-space minimum tiling path BAC clones were sequenced as combinatorial pools by Illumina short-read technology and, after quality trimming of de-convoluted reads, were assembled using Velvet version 1.2.09 as previously described13.
Minimum tiling path BAC clones assigned to different barley chromosomes were sequenced at one of four sequencing centres, relying on highly multiplexed paired-end and mate-pair sequencing libraries using either the Roche 454 Titanium or the Illumina MiSeq, HiSeq2000 and HiSeq2500 platforms (Supplementary Note 1 and ref. 51).
