Sentence examples for minimum tiling path from inspiring English sources
Nine BACs were required to span the contig in a minimum tiling path (Fig. 1); eight tiled BACs were selected for GS FLX pyrosequencing and the final (ninth) BAC was sequenced using standard Sanger sequencing of a shotgun library.
The remaining 73,070 BACs were selected from a minimum tiling path representing the physical map of the barley genome16.
This comprised 15,661 so-called gene-bearing BAC clones, preselected mainly by overgo-probe hybridization for the presence of transcribed genes and fingerprinted for definition of a minimum tiling path of the barley gene space.
These gene-space minimum tiling path BAC clones were sequenced as combinatorial pools by Illumina short-read technology and, after quality trimming of de-convoluted reads, were assembled using Velvet version 1.2.09 as previously described13.
Sources of clones in the minimum tiling path.
In this project, the consortium employed a clone-by-clone sequencing strategy after construction of a minimum tiling path (MTP) for each chromosome.
A minimum tiling path of BACs across the FPC contigs was divided into six regions (each approximately 3 Mb) from which six pools of BACs were created.
Clones that are 90 100% repetitive can be barred from a minimum tiling path for sequencing or during assembly of sequenced data.
As a pilot experiment, we sequenced the 18 Mb Chr3S from O. barthii (the wild progenitor of O. glaberrima) using six pools of 28 BACs (each pool ∼3 Mb) selected from the BAC minimum tiling path.
CG14 BAC library [29] that constitute the minimum tiling path (MTP) established around S1 [27].
