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For each sample, minimum three images were analyzed.
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For each cell line and treatment, a minimum of three images were taken and within each image a minimum of six measurements of distinct mitochondrial regions were taken to give n=18.
Essentially, these methods require recording of minimum three structured images corresponding to precise phase shifts of the grid.
We obtained a minimum of three images.
A minimum of three images for each sample was captured, and representative images were included in Figure 1.
For quantification of colocalization signals, intensity correlation coefficients (ICQ, Li et al. 2004) were obtained using the ImageJ colocalization plug-in (Abramoff et al. 2004) for a minimum of three images per animal.
A minimum of five images were taken from each slides.
A minimum of ten images were captured for each tissue section.
Both average and standard deviation were calculated after measurement of a minimum of nine images corresponding to three independent experiments.
A minimum of five images were acquired in every region over a series of time points spanning 30 min after EtBr application.
For quantitative analysis, EC nuclei number, tight junction number, pericyte cell body/process length, and EC surface length were quantified using ImageJ software (NIH) on a minimum of six images of cerebral blood vessels per sample.
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